Detection of Tomato Spotted Wilt Virus with Elisa

Tomato spotted wilt virus

Tomato spotted wilt virus.

Growers in Georgia first noted problems in their peppers and onions during the last week of March 1989. Thrips populations combined with further field symptoms and the verification of the disease triggered a massive insecticide spray program in Georgia. Monitor was first used in early May followed by combinations of Orthene, Cygon and Thiodan. Most fields had less than 1% infection prompting a ...

Tomato Spotted Wilt Virus on Peanuts

I the last decade, tomato spotted wilt virus (TSWV) has become widely distributed across Alabama on many field, vegetable, and floral crops. About 300 plant species, mainly broadleafs, are hosts of TSWV. In addition to peanut, other important crops grown in the Southeast that are susceptible to this virus include tomato, pepper, potato, cucurbits, lettuce, eggplant, and tobacco. Chrysanthemum, ...

Comparison of ELISA and RT-PCR assays for the detection of Tomato spotted wilt virus in peanut

Diagnosis of Tomato spotted wilt virus (TSWV) in peanut can be accomplished by enzyme-linked immunosorbent assay (ELISA) or reverse transcription polymerase chain reaction (RT-PCR) but there has been no report of a direct comparison of the success of the two assays in evaluating infection rates of field-grown peanut. We collected peanut root samples from field-grown plants, 76 in 2006 and 48 in...

Serological differentiation of 20 isolates of tomato spotted wilt virus.

Twenty tomato spotted wilt virus (TSWV) isolates were serologically compared in ELISA employing five different procedures using a rabbit polyclonal antiserum against nucleocapsid proteins (NuAbR) and mouse monoclonal antibodies (MAbs), two directed to nucleocapsid proteins (N1 and N2) and four directed to glycoproteins G1 to G4. All the antisera were raised against TSWV-CNPH1. The 20 isolates w...